DNA typing of HLA-I antigens with medium resolution method by DNA chip technique
- VernacularTitle:HLA-I类抗原的组织配型基因芯片的建立及应用
- Author:
Gang GUO
;
Rui ZHANG
;
Pei SUN
;
Mingxin ZHANG
;
Dongchun LIANG
- Publication Type:Journal Article
- Keywords:
HLA-I antigens;
Gene chip;
Medium resolution;
Typing
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a DNA typing method for HLA-I antigens with medium resolution method by DNA chip technique.Methods:The chip was made with specific medium distinguish-typing probes designed according to gene frequency of HLA-I alleles from Northern Chinese. Unsymmetrical PCR was used to amplify HLA-I exon2,3,and then the PCR products labeled and hybridized with probes on the chip.Typing of HLA-I was certified by scanning the hybridizing signals of through a set of computer software.Results:HLA-I alleles were successfully typed in 30 clinical samples .This medium-distinguishing probes were able to discern 57 HLA-I alleles accurately.Conclusion:DNA typing of HLA-I by chip has been proven to be a high-resolution and high-specific method. It is able to check out the new alleles that can not be distinguished by other methods with the same resolution., and it is more intuitional and more suitable for clinical application .
