Expression and significance of GSK-3 and P70S6K in rat muscles with insulin resistance
- VernacularTitle:GSK-3及P70S6K在胰岛素抵抗大鼠肌肉中的表达及意义
- Author:
Yanjun WANG
;
Sheng ZHAO
;
Guoliang LIU
- Publication Type:Journal Article
- Keywords:
Glycogen synthase (GSK)-3;
Ribosome S6 protein kinase (P70S6K);
Insulin resistance (IR);
Signal transmission
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe expression and diversity of glycogen synthase (GSK)-3 and ribosome S6 protein kinase (P70S6K) in skeletal muscle of insulin resistance (IR) rat induced by high fat food and to approach the effect and significance of GSK-3 and P70S6K in IR occurrence.Methods:Forty Wistar rats aged four-week were randomly divided into normal control, high-glucose, high-fat and high-fat high-glucose groups. 8 weeks after feeding, insulin sensitivity of the rats was evaluated by hyperinsulinism euglycaemic clamps technique (ring clamp experiment), GSK-3 and P70S6K in rat skeletal muscle were measured by Western blot and epididymis fat pad weight, blood glucose, insulin, triglyceride (TG), cholesterin (TC), free fatty acids (FFAs) and hsCRP levels were also determined.Results:IR was induced, body weight (P
