Adverse Effects of Rotenone on Ventral Mesencephalic Neurocytes of Rats in Culture
- VernacularTitle:鱼藤酮对大鼠中脑原代培养细胞的毒性
- Author:
Mingwei WANG
;
Yanyong WANG
;
Ping GU
- Publication Type:Journal Article
- Keywords:
Rotenone;
Mesencephalic;
Dopaminergic neuron;
Neurotoxicity
- From:
Journal of Environment and Health
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the neurotoxicity induced by rotenone to the primary cultured ventral mesencephalic dopaminergic neurons. Methods SD rat ventral mesencephalic neurocytes were cultured and identified by tyrosine hydroxylase (TH) and glial fibrillary acid protein (GFAP). Immunohistochemistry and morphological observation were used to evaluate the toxicity of rotenone to the dopaminergic neurons and astroglia. Results After 24 h of exposure to rotenone at 0.10 and 1.00 ?mol/L, the number of surviving dopaminergic neurons significantly decreased. The number of surviving astrocytes significantly decreased treated with 1.00 ?mol/L. Exposed to 0.10 ?mol/L rotenone, the morphological change of the dopaminergic neurons was seen, the area of cell body decreased significantly. Conclusion The dopaminergic neurons is more susceptible to the neurotoxicity of rotenone.
