Determination of Methyl Tertiary Butyl Ether in Cerebrospinal Fluid of Rats by Gas Chromatography
- VernacularTitle:大鼠脑脊液中甲基叔丁基醚的气相色谱测定法
- Author:
Yun ZHANG
;
Wenjing LUO
;
Ting YAO
- Publication Type:Journal Article
- Keywords:
Brain;
Methyl tertiary butyl ether;
Chromatography, gas;
Cerebrospinal fluid
- From:
Journal of Environment and Health
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of methyl tertiary butyl ether (MTBE) in the cerebrospinal fluid of rats by gas chromatography and to know whether MTBE can penetrate the blood brain barrier. Methods 9 male SD rats were randomly divided into 3 groups and then exposed to MTBE by gavage at a dose of 1000 mg/kg?bw and by inhalation at a dose of 8 ml/L for 30 days. The cerebrospinal fluid was collected at 6 h, 12 h and 24 h respectively at the end of treatment and the MTBE in rats cerebrospinal fluid was determined by gas chromatography. Results MTBE was detected in the cerebrospinal fluid of rats after subchronic exposure of MTBE through gavage and inhalation. Conclusion MTBE is able to penetrate the blood brain barrier and reach the brain.
