Determination of Borax in Food by RP-HPLC
- VernacularTitle:食品中硼砂的反相高效液相色谱测定法
- Author:
Yan CHEN
;
Hongguang LI
- Publication Type:Journal Article
- Keywords:
Chromatography, liquid;
Borax;
Food
- From:
Journal of Environment and Health
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method for determination of borax in food by RP-HPLC. Methods Borax in food was extracted by acid methanol followed by turmeric acetic acid derivation and then detected by HPLC. Symmtry C18(150 mm?4.6 mm, 5 ?m) was used as the stationary phase, methanol-water (80+20) as the mobile phase, the detection wavelength was 550 nm. Results The detection limit was 6.4 ng/kg, the relative error was 2.3%, the recovery rates were between 90.63% and 96.88%, the liner range of the borax was 0.032-0.32 ?g/ml, the regressive equation of calibration curve was y=3 210 000x+121 000, r=0.999 2. Conclusion The present method is simple, rapid, accurate with satisfactory results.
