Effects of bFGF on the proliferation of human salivary adenoid cystic carcinoma ACC-2 cell line and MEK/ERK, NF-?B signaling pathway
- VernacularTitle:bFGF对人涎腺腺样囊性癌ACC-2细胞株增殖及MEK/ERK、NF-?B通路的影响
- Author:
Lei DING
;
Shengrong ZHU
;
Guolin XIANG
- Publication Type:Journal Article
- Keywords:
Basic fibroblast growth factor;
Adenoid cystic carcinoma;
Extracellular signal-regulated kinase;
NF-?B
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of bFGF on the proliferation of human salivary adenoid cystic carcinoma cell ACC-2 in vitro. Methods:The effect of bFGF on proliferation of ACC-2 cell line was observed by MTT assay; ERK activity was measured by immuno-precipitation; and ERK and I-?B? expressions were assessed by Western blot. Results:bFGF can enhance the proliferation of ACC-2 cell line. Stimulated by bFGF, the proliferation ratio increased significantly. The intracellular ERK activity and p-ERK expression were increased and I-?B? expression was inhibited by different concentrations of bFGF. The above effects of bFGF can be attenuated by MEK inhibitor U0126. Conclusion:bFGF stimulates the proliferation of ACC-2 in the dose dependent manner, which may be due to up-regulating ERK, NF-?B signaling pathway.
