Effects of Ulinastatin on Renal Apoptosis and Expression of bcl-2 in Rats with Severe Acute Pancreatitis
- VernacularTitle:乌司他丁对重症急性胰腺炎大鼠肾脏细胞凋亡及bcl-2基因表达的影响
- Author:
Yongjing ZHUANG
;
Yongyang YU
;
Guirong WU
- Publication Type:Journal Article
- Keywords:
Severe acute pancreatitis Kidney Cell apoptosis Ulinastatin
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of ulinastatin(UTI) on renal apoptosis and expression of bcl-2 in rats with severe acute pancreatitis(SAP).Methods Sixty rats weighing 250-300 g were randomized divided into 3 groups:pseudo-operation group(SO group,n=20),SAP group(n=20) and UTI treated group(UTI group,n=20).The model of SAP was established by retrograde injection of 5% sodium taurocholate solution into the biliopancreatic duct in the rats.Serum Cr and BUN were determined.The left kidneys were resected for light and electronic microscopic study.Renal cell apoptosis was determined by TUNEL.Expression of bcl-2 was detected by immunohistochemical staining of SABC.Results Serum Cr,BUN,renal cell apoptotic index and bcl-2 expression were markedly increased in SAP group compared with SO group(P
