Evaluated on Expression of Endothelin-3 mRNA Detected by RT-PCR in Acute Pancreatitis Rats
- VernacularTitle:急性胰腺炎大鼠内皮素-3基因表达的逆转录检测
- Author:
Yong ZHANG
;
Yongzhang GUO
;
Li LI
;
Xinan CAO
- Publication Type:Journal Article
- Keywords:
Endothelin-3 mRNA Pancreatitis Microcirculation
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between gene expression of endothelin-3(ET-3) and inflammation of acute pancreatitis(AP) in rats.Methods Fifty-four rats were divided randomly into 4 groups:sham operation group,AP group,arterial injection group and vein injection group.AP was induced by reverse intra-bile duct infusion 4.5% sodium taurocholate,treated with low dose dopamine 〔5 ?g/(kg?min)〕 by injecting arterial or tail vein.Rats were sacrificed at 1,6 and 24 h after the induction of AP.The mRNA expression of ET-3 was evaluated by semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR) and pathological changes was observed in rats.Results Expression of ET-3 mRNA could be detected from 1 up to 24 h after the induction of pancreatitis.Expression of ET-3 mRNA of sham operation group was decreased significantly compared with other three groups.Expression of ET-3 mRNA showed a significant decrease by arterial injection dopamine than that by tail vein(P
