Establishment of Hepatocellular Carcinoma Cell Lines with Stable Decreased Expressing IGF1R Gene with siRNA
- VernacularTitle:运用siRNA建立稳定低表达IGF1R基因的肝癌细胞株的实验研究
- Author:
Jian NIU
;
Xiangnong LI
;
Zeguang HAN
- Publication Type:Journal Article
- Keywords:
Human insulin like growth factor-1 receptor Small interfering RNA Hepatocellular carcinoma Gene transfection Stable expression
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish hepatocellular carcinoma(HCC) cell lines which olig-expressed IGF1R gene stably.Methods An eukaryotic expressing vector pSUPER-IGF1R-siRNA that could block IGF1R expressing was transferred into hepatocellular carcinoma cell lines SMMC7721 and Hep3B with Lipofectamine 2000 reagents.After transferred,cells were selected with G418 to obtain positive clones.The expressions of IGF1R,cyclin D1 and cyclin B1 were detected by RT-PCR and Western-blot.Cell growth curve were painted.Results Two cell lines clones were screened olig-expressing IGF1R gene stably.The experimental cell lines grew more slowly than control cell lines and the expression of cyclin D1 decreased(P
