The purification of anti-D antibody from IgG contained anti-D by the RhD positive red blood cell
- VernacularTitle:用RhD阳性红细胞从含抗D抗体的IgG中提纯抗D抗体
- Author:
Mingjun SHI
;
Jing XIE
;
Jiang ZHONG
;
Huazhen GUI
;
Bing GUO
;
Guozhong ZHANG
- Publication Type:Journal Article
- Keywords:
Anti-D antibody;
IgG;
Red blood cell;
Column ion-exchange chromatography;
Affinity chromatography
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the purified methods of human anti-D antibody from IgG contained anti-D. Methods:The IgG was separated by the column ion-exchange chromatography(CIEC) from the plasma in which the content of anti-D was 0.814 ?g/ml. Then the IgG preparation contained anti-D was purified by the affinity chromatography(AC) with the O group, RhD positive red blood cell (genotype CCDee). Results:The content of non anti-D IgG were reduced about 90% by the method of AC and the proportion of anti-D could be significantly increased in the final preperation. The quality of final preparation attained reqirements of national standard of biologics. Conclusion:This method is able to purify anti-D from IgG contained anti-D and offer a reference for plasma products.
