The effect of interferon-? on bleomycin-induced pulmonary fibrosis and expression of interferon-? and transforming growth factor-? in rats
- VernacularTitle:干扰素?对实验性大鼠肺纤维化、肺组织干扰素?和转化生长因子?表达的影响
- Author:
Deping ZHANG
;
Yi ZHUANG
;
Fanqing MENG
;
Kaisheng YIN
- Publication Type:Journal Article
- Keywords:
Interferon-?;
Transforming growth factor-?;
Pulmonary fibrosis
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of interferon-? on bleomycin-induced pulmonary fibrosis in rats and to study the mechanism of its effects.Methods:60 SD rats were randomly divided into 3 groups of 20:normal control group(Group N), model control group(Group M) and interferon-?(IFN-?)treated group(Group R). The pulmonary fibrosis model was established by a single intratracheal injection of 5 mg/kg of bleomycin. The rats were sacrificed at days 3,7,14,28 after modeling in batch. Lung tissues were restored to analyze the pathological changes, the content of hydroxyproline,mRNA expression of IFN-? and transforming growth factor-?(TGF-?).Results:The scores of alveolitis in Group M and R were higher than that in Group N at each poin-in-time after modeling.The scores of alveolitis in Group R were higher than that in Group M. The scores of pulmonary fibrosis and the content of hydroxyproline in lung tissues in Group M and R were higher than that in Group N at day 14 and 28. The expression of IFN-? mRNA in lung tissues at day 3,7,14,28 and TGF-? mRNA at day 3 in Group R were higher than that in Group M(P
