Effects of High Levels Iodine Intake through Meal on mRNA Expression of Placental and Breast NIS in Rats
- VernacularTitle:高碘摄入对大鼠乳腺和胎盘钠碘转运体mRNA表达的影响
- Author:
Laixiang LIN
;
Yina SUN
;
Jinru DONG
- Publication Type:Journal Article
- Keywords:
Iodine;
Placenta;
Breast;
Na+-I-symporter
- From:
Journal of Environment and Health
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of excessive iodine intake through the meal on the expression of mRNA of placental NIS in pregnant rat and breast NIS in lactating rats. Methods Wistar rats, weaning one month, were randomly divided into three groups according to the body weights, i.e., normal iodine (NI), ten fold high iodine(10HI), one hundred fold high iodine(100HI), the ratio of female and male was 2∶1. Iodine intake of the groups were about 6.15, 61.5 and 615.0 mg/d respectively. After 3 months of treatment, the urine iodine was determined by As-Ce-catalytic spectrophotometry. The rats mated and had offspring. Their placenta and breasts were taken on the seventeenth day of pregnancy and tenth day of lactation respectively. Then NIS mRNA expression was determined by RT-PCR. Results The urine iodine increased with the increase of the iodine intake. The urine iodine and iodine intake showed the parallel magnification. Compared with the NI group,AR value of the placental NIS mRNA in 100HI group and the breast NIS mRNA in 10HI and 100HI group significantly decreased. Conclusion Excessive iodine intake may down-regulate the expression of the placental and breast NIS, which presents a protective effect on offspring.
