Fermentation Technology Optimization of Flocculant-producing Strain B5
- VernacularTitle:产絮凝剂菌株发酵工艺的优化
- Author:
Zhaohui WANG
;
Hui YE
;
Yan CHANG
- Publication Type:Journal Article
- Keywords:
Flocculations;
Flocculating-producing strain;
Optimum media
- From:
Journal of Environment and Health
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the optimum component proportion of media and the best fermentation conditions for the flocculatnt-producing strain B5. Methods Through single factor experiment to seek the best carbon sources, nitrogen sources and inorganic salts. Furthermore, through orthogonal test to select the best conditions for producing flocculants by strain B5. Results The best carbon source, nitrogen source and inorganic salt were glucose, (NH4)2SO4 and CaCl2, the content of glucose was 2.0%, the initial pH value of medium was 7.0, the temperature was 30 ℃, agitation rate was 160 r/min and culture time was 24 hours in flakes, 50 ml liquids (250 ml triangular flask), the inoculation volume was 0.2 ml. The flocculants produced by strain B5 under the best fermentation conditions has been furnished for multifarious waste water, the flocculating activities were from 88.4% to 97.2% and reached to the highest on the waste water of dairy farming. Conclusion The flocculants produced by the strain B5 is applicable to the waste water treatment.
