Experimental study of BMP-2 derived peptide on osteogenic induction in BMSC in vitro
- VernacularTitle:BMP-2活性多肽体外定向诱导大鼠BMSCs向成骨方向分化的实验研究
- Author:
Zhixia DUAN
;
Qixin ZHENG
;
Xiaodong GUO
- Publication Type:Journal Article
- Keywords:
BMP-2-derived peptide;
BMSCs;
osteoinductivity
- From:
Orthopedic Journal of China
2006;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the capability of synthesis BMP-2-derived peptide on osteogenic induction in bone marrow stem cells(BMSEs),and to evaluate the osteoinductivity of BMP-2-derived peptide in vitro.[Method]After segregating and cultivating four weeks Wistar rats marrow stromal cells in induction group were induced by osteogenasis medium containing 200 gg/ml BMP-2-derived peptide,and in non-inductional group BMSCs were still culture with DMEM medium.Following continue culture for 2~4 weeks,cells film preparation,alkaline phosphatase activity and calcium deposition were measured.Type I collagen(CoM)and osteopontin(OPN)mRNA expression were measured using real-time fluorescent quantitative polymerase chain reaction(FQ-PCR)technique.The capability of synthesis BMP-2-derived peptide on osteogenic induction of BMSCs was investigated.[Result]After inductived with BMP-2-derived peptide.BMSCs cultured survived well greatly changed in cell morphology,and showed a biological and morphologie characteristics similar to those of osteoblasts.The lever of alkaline phosphatase activity and calcium deposition increased.Col-Ⅰand OPN mRNA were expressed at higher level.In non-inductional group no conspicuous osteogenic induction was shown.[Conclusion]BMP-2-derived peptide can induce BMSCs to differentiate into osteoblasts.It has the similar capacity of osteogenic induction as nature BMP-2,so BMP-2-derived peptide is an ideal cell agent for bone tissue engineering,and can be available widely.
