Screening of human anti-ricin ScFv from large phage library
- VernacularTitle:从大容量抗体库中筛选抗蓖麻毒蛋白抗体
- Author:
Yuanyuan QIAO
;
Yuxiao WANG
;
Lijun ZHOU
;
Yu ZHAO
;
Yuxia WANG
;
Xiaohang ZHAO
;
Yan WANG
- Publication Type:Journal Article
- Keywords:
Large phage library;
Ricin toxin;
Phage antibody library;
Soluble antibodies
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To clone human anti-ricin antibodies from large phage antibody library.Methods:Panning for a large phage library against ricin toxin was conducted to select specific antibodies against ricin. The binding activities and specificities were tested by ELISA method. Soluble ScFvs were prepared through infecting E coli. HB2151 with the selected phage antibodies and induction with IPTG. Results:Forty positive clones were obtained after 5 rounds of panning, and 12 clones had specific binding ability to ricin toxin. DNA fingerprinting showed 7 different band patterns indicating 7 different positive clones. DNA sequencing showed that variable regions of these ScFvs belonged to different subgroups.Conclusion:Human anti-ricin antibodies were successfully obtained from large phage antibody library.
