The effect of cigarette smoke extract on ICAM-1 expression as well as the influence of adenovirus E1A gene in pulmonary epithelial cells
- VernacularTitle:香烟提取物和腺病毒E1A基因对肺泡上皮细胞ICAM-1表达的影响
- Author:
Yiqing QU
;
Wei XIAO
;
Shaoguang HUANG
;
Huanying WAN
- Publication Type:Journal Article
- Keywords:
Cigarette smoke extract(CSE);
Adenovirus E1A gene;
Alveolar epithelial cell;
ICAM-1
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of intercellular adhesion molecule-1(ICAM-1 )induced by cigarette smoking extract(CSE) as well as the influence of the adenovirus E1A gene in pulmonary epithelial cells.Methods:A549 cells were transfected with a plasmid carrying the adenovirus E1A gene and stable trasfectants expressing E1A protein were selected. The cells were tested for the expression of ICAM-1 after stimulation with different concentrations of CSE.Results:ICAM-1expression was increased in A549 markedly with CSE stimulation,and the higher CSE concentration were,the more expression of ICMA-1 was observed. As compared with parental cells or cells transfected with control plasmid, ICAM-1 expression was markedly increased with stimulation of different concentrations of CSE in E1A-positive A549 cells.Conclusion:CSE exposure induces the surface expression of ICAM-1 and adenovirus E1A gene can markedly increase ICAM-1 expression induced by CSE in pulmonary epithelial cells, which suggest that latent adenovirus infection may amplify the inflammation process present in airways of smokers.
