Research of long peripheral nerve defect repair with artificial nerve composed with Schwann cell
- VernacularTitle:雪旺细胞构建人工神经在大鼠长段神经缺损中的修复作用研究
- Author:
Biao CHENG
;
Bolin LI
;
Zhengrong CHEN
- Publication Type:Journal Article
- Keywords:
artificial nerve;
long defect;
nerve regeneration
- From:
Orthopedic Journal of China
2006;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the effect of repairing of rat peripheral nerve defect with engineering artificial nerve composed with Schwann cells and poly lactic glycoli aced(PLGA).[Method]Human Schwann cells were vaccinated into PLGA tube with polyglactin 910.Sixty SD rats were divided into 3 groups randomly with 20 rats in each one: group A,self-nerve graft;group B,Schwann cells artificial nerve;group C,pure PLGA.Evaluations of electrophysiological and histological examines were carried out 8 weeks ad 12 weeks after operation.[Result]The outcome of group B showed similar to group A in general and histological study,however it expressed slightly lower in electrophysiological study,muscle weight and nerve fiber quantity.Marked neuron could be found in spinal cord after group B had been traced.Large number of regeneration nerve fibers could be seen from electron microscope.[Conclusion]The tissue engineering artificial nerve composed with Schwann cells is able to repair 20 mm defects of peripheral nerve,and it has an approximate treatment effect to the self-nerve grafting.
