Adhesion of bone marrow mesenchymal stem cells cultured on PLGA modified with Ⅱ collagen
- VernacularTitle:骨髓间质干细胞与Ⅱ型胶原修饰的PLGA黏附性的观察
- Author:
Li ZHANG
;
Zhengang ZHA
;
Changren ZHOU
- Publication Type:Journal Article
- Keywords:
bone marrow mesenchymal stem cell;
PLGA;
tissue engineering
- From:
Orthopedic Journal of China
2006;0(20):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore a method for isolating and culturing bone marrow mesenchymal stem cell(BMSCs) in vitro,and improve the cellular conglutination ability of biomaterial poly-lactide-co-glycolic acid(PLGA),and observer the adhesion of BMSCs cultured on PLGA modified with Ⅱ collagen.[Method]BMSCs were isolated and cultured in vitro.The cell exterior antigen,cell livingness and cell cycle were analyzed by flow cytometry method,and cellular configuration was observed continually under invertd phase-contrast microscope.PLGA was made by phase separation,and was modified with Ⅱ collagen.The third era BMSCs were cultured on PLGA,and its adhesion with biomaterial was observed scan electron microscope.[Result]BMSCs could be isolated and cultured in vitro,and express CD29,CD44,CD106,but not express CD34,CD45.The cell livingness was 88.96%,cells in G0-Glperiod are 90.32%.The cell morphology was spindle.The average diameter of PLGA modified with Ⅱ collagen was 100 ?m,and PLGA has good adhesion with BMSCs.[Conclusion]BMSCs could be cultured stability in vitro for long time,and is good seminal cell of tissue engineering.Ⅱ collagen can improve the cellular adhesion of PLGA,PLGA modified with Ⅱ collagen is the good biomaterial of tissue engineering.
