Comparison of the adherent cells derived from human placenta,umbilical cord blood and bone marrow
- VernacularTitle:胎盘、脐血和骨髓来源的贴壁细胞的生物学特性的比较
- Author:
Xin LIU
;
Boling LI
;
Bo XU
- Publication Type:Journal Article
- Keywords:
placenta;
umbilical cord blood;
mesenchymal stem cell
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the adherent cells derived from human placenta,umbilical cord blood and bone marrow,and provide laboratory data for clinical application of mesenchymal stem cells.Methods Adherent cells were isolated from human placenta tissues by enzyme digestion,and mononuclear cells(MNC) were isolated from umbilical cord blood(UCB) by 60g/L HES and density gradient centrifugation and MNC were isolated from bone marrow(BM) by density gradient centrifugation,and then these cells were cultured in vitro.Their biological characteristics were studied and compared.Results The adherent cells cultured from human placenta and umbilical cord blood had disparate shape in vitro respectively.And they had some differences in growth and shape from those derived from bone marrow.The adherent cells derived from the three tissues all expressed CD106 and CD44 in immunohistochemistry staining.Conclusion The adherent cells derived from human placenta and umbilical cord blood have the basic features of mesenchymal stem cells.
