Application of laser capture microdissection to capture pure cells in lung cancer and their paired normal lung tissues
- VernacularTitle:应用激光捕获显微切割技术制备肺癌蛋白质组学研究样品
- Author:
Yingxuan TIAN
;
Yandong NAN
;
Shuanying YANG
- Publication Type:Journal Article
- Keywords:
laser capture microdissection;
lung cancer tissue;
proteomics
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Microheterogeneity is an important problem in molecularly biological and proteomic research on malignancies.The goal of this study was to investigate the method to purify targeted cells in lung cancer and its paired normal tissues for proteomic study on lung cancer applying laser capture microdissection(LCM).Methods LCM technique was emploged to obtain the cells of lung cancer tissues and their paired normal tissues from frozen sections.Results LCM can be applied to quickly and precisely obtain pure targeted cells subgroup or single cell without interstitium under the microscope.Conclusion LCM can tackle the problem of tissue heterogeneity in molecular analysis successfully.These results indicate that LCM has a potential as a tool in lung cancer proteomic research.
