Effects of Tiam 1 antisense oligodeoxynucleotides transfection on the invasive and migratory potentials of gastric cancer cells in vitro and vivo
- VernacularTitle:Tiam 1反义寡核苷酸转染对胃癌细胞体内外侵袭转移能力的影响
- Author:
Jinming ZHU
;
Peiwu YU
;
Yongliang ZHAO
- Publication Type:Journal Article
- Keywords:
T lymphoma invasion and metastasis inducing factor;
gastric cancer;
antisense oligodeoxynucleotides;
invasion and metastasis
- From:
China Oncology
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:As a regulatory factor of cytoskeletal structure,a close correlation existed between Tiam 1(T lymphoma invasion and metastasis inducing factor 1) and the invasion and metastasis of gastroenteric tumor.We aimed to observe the effects of Tiam 1 antisense oligodeoxynucleotides(Tiam 1 ASODN) transfection on the invasive and migratory potentials of gastric cancer cells in vitro and vivo.Methods:The higher invasive and migratory subgroup(MH) were separated from human gastric cancer cell line MKN-45(M0) by laminin adhesion method in vitro.Tiam 1 ASODN was transfected into MH cells with liposome,and the expression of Tiam 1 mRNA and protein was determined by RT-PCR and ELISA separately.Changes in the invasive and migratory potentials of MH cells in vitro and vivo after transfected with ASODN were observed by Boyden chamber test and inoculation into nude mice respectively.Results:The expression of Tiam 1 mRNA and protein in MH cells after transfected with 0.43 ?mol/L ASODN(0.162?0.018,0.982?0.119) was significantly lower than that of either Liposome-transfected cells(0.789?0.054,1.237?0.108),sense oligodeoxynucleotides(SODN)-Liposome-transfected cells(0.754?0.039,1.234?0.103) or No-transfected cells(0.801?0.065,1.290?0.182)(P
