Biological effects of connective tissue growth factor transfection on human breast cancer cells
- VernacularTitle:结缔组织生长因子转染对人乳腺癌细胞系生物学行为的影响
- Author:
Min LU
;
Jiangfeng YOU
;
Jieliang WANG
;
Xianglin CUI
;
Jie ZHENG
- Publication Type:Journal Article
- Keywords:
connective tissue growth factor;
breast cancer;
apoptosis;
tumor invasion
- From:
China Oncology
2006;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Connective tissue growth factor(CTGF) is a member of CCN family,it has been reported that CTGF involve in many biological processes and various pathological conditions.In our study,the correlation of CTGF expression and biological effects on breast cancer cell lines were investigated.Methods:The eukaryotic expression vectors containing CTGF open reading frame(ORF) pcDNA3.0/CTGF were constructed and transfected into breast cancer cell lines.The relationship between CTGF expression and breast cancer cell growth ability and proliferation,cell cycle distribution,apoptosis,cell motility and invasive capacity in vitro were observed.Results:Upregulation of CTGF expression in MCF-7 cell line could inhibit its growth ability and proliferation,increased the proportion of G0G1 phase cells,enhanced apoptosis and inhibited its invasive ability in vitro.Downregulation of CTGF expression in MDA-MB-231 cell line increased its growth ability and proliferation,decreased apoptosis and promoted its invasive ability in vitro.There were no differences of cell motility among different groups in MCF-7 and MDA-MB-231 cell lines.Conclusions:CTGF can inhibit breast cancer cell growth by increasing cell apoptosis and/or the proportion of cells in G0G1 phase.CTGF can also inhibit breast cancer cell invasive ability.
