Experimental studies of optimized transfection condition on human malignant melanoma cell by survivin antisense oligonucleotide
- VernacularTitle:反义寡核苷酸转染A375细胞实验条件的优化
- Author:
Mei WANG
;
Bing WANG
;
Shengshun TAN
- Publication Type:Journal Article
- Keywords:
survivin;
malignant melanoma;
antisense oligonucleotide(ASODN);
liposome;
cell culture
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effects of survivin antisense oligonucleotide on proliferation of human malignant melanoma cell in vitro in different transfection condition.Methods By using trypan blue exclusion method,this study screened optimized transfection condition that inhibited A375 cell viability.Results ① By the phosphorothiote-modification and the liposome-encapsulation,there were significant differences in ASODN with ASODNL.ASODNL were stronger in inhibiting A375 cell proliferation(P
