Role of NF-?B p50 protein in pathologic responses of experimental acute pancreatitis in mice
- VernacularTitle:NF-? Bp50基因缺失对小鼠实验性急性胰腺炎的影响
- Author:
Gang ZHAO
;
Meixiong HUANG
- Publication Type:Journal Article
- Keywords:
Nuclear factor ?B?p50? Acute pancreatitis?Necrosis?Apoptosis
- From:
Chinese Journal of Current Advances in General Surgery
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the roles of p50 protein in pathologic responses of cerulein(CR)pancreatitis in mice deficient in p50 protein of NF-?B.Methods:Pancreatitis was induced by i.p.injection of 50?g/kg CR.The express and compensation of p65,p50 and cRel proteins of NF-?B,NF-?B activity were measured using electromobility shift assay;parameters of pancreatitis,eg,amylase,lipase and trypsin were evaluated.The apoptosis,necrosis and caspase-3 activity were observed.Results:p50 knockout(KO)mice had no p50 protein expression and increased p65 and cRel proteins expression.p50 KO mice's NF-?B activity decreased and apoptosis increased in pancreatitis compared with wild type(WT)mice.There was no caspase-3 activition in both mice before and after pancreatitis.All KO mice had an increased necrosis and amylase,lipase,trypsin activity compared with WT mice in pancreatitis.Conclusions:The results indicated that NF-?B p50 genetic deletion might attenuate cerulean induced pancreatitis.
