Investigation on Perioperative Renal Aquaporin 2 Expression in Experimental Obstructive Jaundice
- VernacularTitle:梗阻性黄疸围手术期肾髓质水通道蛋白2表达变化的实验研究
- Author:
Yong WANG
;
Jilong HAN
;
Jingang LIU
- Publication Type:Journal Article
- Keywords:
Obstructive jaundice Aquaporin 2 Perioperative period
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of renal medulla aquaporin 2 expression and morphological changes of epithelia of collecting tube after bile duct recanalizaiton operation.Methods Thirty rats were divided into two groups randomly.Common bile duct ligation was performed on 20 experimental rats with silicon tubes 2 mm in extre-diameter,and sham operation on the other 10 rats.Seven days later,bile duct recanalizaiton was performed on obstructive jaundice group and sham operation on contrast group.Experimental rats were divided into two subgroups randomly.Half of them were killed immediately and the others would be killed 24 hours later.Serum of each rat was collected to detect hepatic function and renal function.Renal medulla was fixed for microscopic examination and was kept in the-80 ℃ refrigerator for aquaporin 2 expression measurement by Western blot technique.ResultsAll of the animals accomplished the experiment smoothly.Golden ascites were found in the rats of obstructive jaundice group.Twenty-four hours after recanalization,serum bilirubin levels decreased 〔(45.95?8.39)?mol/L〕,P
