Study on recombinant chicken ? interferon production in yeast Pichia pastoris and detection for its antiviral activity
- VernacularTitle:重组酵母鸡?干扰素的研制及其抗病毒活性测定
- Author:
Meihong CAI
;
Ruibing CAO
;
Chuanyou WANG
;
Jie SHENG
;
Lulin PAN
;
Puyan CHEN
- Publication Type:Journal Article
- Keywords:
Chincken interferon ?(ChIFN-?);
Secreted expression;
Anti-virus
- From:
Chinese Journal of Immunology
2000;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain eukaryotic expressing protein of chicken interferon ? (ChIFN-?) and research its anti-virus activity.Methods: Chicken interferon ? mature protein gene was cloned and amplified by reverse transcripition-polymerase chain reaction(RT-PCR) from the total mRNA in the lymphocyte of chicken blood stimulated with ConA for 4～10 hours.The gene was inserted into the expression vector pPICZa-A,which had been cleaved by EcoR I and Xba I.The recombinant vector was linearized by Sac I and transferred into yeast Pichia pastoris,strain X33,anti-virus activity of the recombinant cytokine was detected by the classical experiment cell pathological effect inhibition assay.Results:The result showed that the preparation of recombinant interferon had higher anti-virus activity(10?48 U/ml).Conclusion: The recombinant chicken interferon ? with anti-virus bioactivity has been obtained.
