Effects of Fluoride on Cell Cycle, Apoptosis and NF-?B Expression in Cultured Rat Hippocampal Neurons
- VernacularTitle:氟对原代培养大鼠海马细胞周期及细胞凋亡和核因子-?B表达的影响
- Author:
Ming ZHANG
;
Ping HE
;
Aiguo WANG
- Publication Type:Journal Article
- Keywords:
Fluoride;
Hippocampal neuron;
Cell cycle;
Apoptosis;
Nuclear factor kappa B
- From:
Journal of Environment and Health
2007;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of fluoride on the cell viability, cell cycle, apoptosis, and expression levels of NF-?B in primary cultured rat hippocampal neurons. Methods The rate of cell survivor, distribution of cell cycle, the percentage of apoptotic cells and the mRNA expression level of NF-?B were respectively measured by methyl thiazolyl tetrazolium (MTT), flow cytometry (FCM), and reverse transcriptase polymerase chain reaction (RT-PCR) after the hippocampal neurons were incubated with 20, 40, and 80 ?g/ml sodium fluoride for 24 hours in vitro. Results Compared with the control group, the rate of cell survivor were significantly decreased in 80 ?g/ml fluoride-treated group (P
