Determination of Malachite Green in Water by High Performance Liquid Chromatography
- VernacularTitle:水中孔雀石绿的高效液相色谱测定法
- Author:
Yingai GUI
;
Lin MA
;
Dan WANG
- Publication Type:Journal Article
- Keywords:
Water;
Malachite green;
High performance liquid chromatography;
Fresh water
- From:
Journal of Environment and Health
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a sensitive HPLC method for the determination of malachite green in fresh water. Methods The HPLC column of ZORBAX SB-C18 (250 mm?4.6 mm, 5 ?m) was employed and acetonitrile and acetate buffer (0.1 mol/L, pH=4.5, V∶V=80∶20) were taken as the mobile phase. The samples were tested at wave length of 588 nm after post-column oxidation with PbO2 flowed at 1.5 ml/min. Results The average recovery rate were 98.5% and 97.8% respectively at the water concentration of 2.0 ?g/L and 10.0 ?g/L,the relative standard deviations (RSD) were 11.2% and 8.0%,the least detection limit was 0.02 ?g/L. Conclusion The method is stable,sensitive and suitable for the detection of malachite green in water.
