The Effect of Lycium Barbarum Polysaccharides on Fibroblast Irradiated by Ultraviolet A
- VernacularTitle:枸杞多糖对长波紫外线辐射暴露的人皮肤成纤维细胞的影响
- Author:
Qiru SONG
;
Faxuan WANG
;
Jin YANG
- Publication Type:Journal Article
- Keywords:
Ultraviolet A;
Lycium barbarum polysaccharides;
Fibroblast
- From:
Journal of Environment and Health
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effects of lycium barbarum polysaccharides(LBP) on fibroblast in vitro irradiated by ultraviolet A(UVA). Methods Taking the primary cultured fibroblast as objects, the fibroblast was irradiated by UVA ( irradiation intensity: 2.4 J/cm2). The fibroblast was randomly divided into six groups, control group, UVA radiated group and four protective groups(0.1 mg/ml LBP, 0.2 mg/ml LBP, 0.4 mg/ml LBP and 0.8 mg/ml LBP). The activities of cell proliferation were measured by MTT methods. The contents of MDA, the activities of SOD in the fibroblasts, and the activities of LDH in the supernatants of fibroblasts were determined by biochemical methods. Results The fibroblasts were irradiated by UVA (irradiation intensity: 2.4 J/cm2),the activities of cell proliferation was decreased,the activities of SOD was decreased too, the content of MDA and LDH increased. Compared with UVA irradiated group,in the given concentration,LBP could improve the activities of cell’s proliferation,improve the activities of SOD and decrease the contents of MDA in the cell, and decrease the content of LDH in the supernatants of cells significantly (P
