Apoptosis of nasopharyngeal cancer cells line CNE2 induced by diallyl disulfide and its possible molecular mechanism
- VernacularTitle:二烯丙基二硫诱导人鼻咽癌CNE2细胞凋亡及其可能的分子机制
- Publication Type:Journal Article
- Keywords: diallyl disulfide; nasopharyngeal carcinoma; CNE2 cells; apoptosis
- From: China Oncology 2006;0(08):-
- CountryChina
- Language:Chinese
- Abstract: Background and purpose:Diallyl disulfide (DADS) is an oil-soluble organosulfur compound found in garlic. It can induce apoptosis in various cancer cell lines in vitro. This study investigated the effect of apoptosis of nasopharyngeal cancer cells line CNE2 induced by diallyl disulfide (DADS) and its molecular mechanisms. Methods:The growth inhibition and apoptosis of CNE2 cells and the protein levels of Bcl-2, Bax and Caspase 3 were examined by MTT assay, light microscopy, flow cytometry, DNA agarose gel electrophoresis and Western blot, respectively.Results:MTT assay showed that 50,100,200,400 ?mol/L DADS significantly inhibited proliferation of CNE2 cells at 48 hr, its inhibition ratio were 3.66%, 14.28%, 29.66%, 61.28%, respectively, in dose-dependent manner (P