Effect of melatonin on calyculin A-induced tau hyperphosphorylation
- VernacularTitle:褪黑素对花萼海绵诱癌素诱导的tau蛋白过度磷酸化的影响
- Author:
Xiachun LI
;
Jianzhi WANG
- Publication Type:Journal Article
- Keywords:
calyculin A;
tau;
melatonin;
hyperphosphorylation;
Alzheimer's disease
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vivo effect of melatonin(Mel) on calyculin A(CA)-induced tau hyperphosphorylation in neuroblastoma cells(N2awt).Methods We treated N2awt cells with CA or CA and 50 ?mol/L Mel,detected the level of tau phosphorylation with immunofluorescence,and assayed the activities of GSK3 and the ratio of GSK-3? phosphorylated at Ser9 site to total GSK-3?.Results CA treatment led to tau hyperphosphorylation accompanied with the increased activity of GSK-3 and the decreased ratio of GSK-3? phosphorylated at Ser9 site to total GSK-3?.When the cells were incubated simultaneously with CA and 50 ?mol/L Mel,the CA-induced tau hyperphosphorylation,GSK-3 activation and the ratio of GSK-3? phosphorylated at Ser9 site to total GSK-3? decrease were attenuated.Conclusion Melatonin protects neuroblastoma cells from CA-induced tau hyperphosphorylation.Its protection may be related to the regulation of GSK-3 activity and the ratio of GSK-3? phosphorylated at Ser9 site to total GSK-3? increase.
