Study on endostatin inhibitation of the growth of transplanted hepatic cancer in nude mice
- VernacularTitle:内皮抑素基因对鼠移植性肝癌抑制作用的实验性研究
- Author:
Jianli WANG
;
Zhaoyang LI
;
Xueying SUN
;
Yunjin ZANG
- Publication Type:Journal Article
- Keywords:
Gene,endostatin ?Hepatic cancer?In vivo
- From:
Chinese Journal of Current Advances in General Surgery
1999;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of endostatin on the growth of human liver carcinoma in vivo. Methods: Mice endostatin gene was transferred into SMMC 7721 cancer established in nude mice. Immunohistochemistry and Western blot analysis were applied to examine the transfection and expression. The microvessel desity (MVD), the positive rate of vascular endothelial growth factor (VEGF) were studied using immunohistochemical methods. The growth of the cancer of the endostatin-transfected were observed. Results: Immunohistochemistry and Western blot proved endostatin expression and secretion from endostatin-transfected SMMC7721 cells, compared with negative control group, MVD and VEGF were lower (P
