Study on diversification of quantity of collagen and proteoglycan and calcium in the posterior longitudinal ligament of the human cervical spine
- VernacularTitle:颈椎后纵韧带胶原、蛋白多糖、钙含量变化的研究
- Author:
Xiaotao WU
;
Yong XU
;
Xiaogang CHEN
- Publication Type:Journal Article
- Keywords:
cervical spondylosis;
posterior longitudinal ligaments;
collagen;
proteoglycan;
calcification
- From:
Orthopedic Journal of China
2006;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the mechanism of posterior longitudinal ligament in cervical spine through measuring the collagen and Proteoglycan and Calcium changes.[Method]Fifteen specimens of cervical longitudinal ligament from cervical spondylotic myelopathy(CSM)and ten control specimens from corpses without cervical spondylosis were obtained.The content of the collagen was measured by Weossner method.Collagen type Ⅰ and Ⅱ were measured by Enzyme-Linked Immunosorbentassay(ELISA)method.Phloroglucinol spectrophotometer to determine the change of amount of Proteoglycan.The Calcium by Methyl-Thymes-Blue(MTB)Colorimetric Method.The specimens were treated hy Hematoxylin and Eosin(HE)stain method and by Masson stain method,the pathological changes of two groups were observed through microscopy.[Result]In CSM,as compared to the control groups,there showed a decrease in the contents of the total Collagen,Collagen type Ⅰ and Proteoglycan,and increase in the content of collagen type Ⅱ.The rate of type Ⅰ/Ⅱ in CSM was lower than that in control groups.An increase in the content of the Calcium.All of which have statistic significance(P
