Correlation between revascularization effect and calcium content of a kind of man-made compound bone on the femoral head defects and necrosis of canines
- VernacularTitle:复合人工骨修复犬股骨头缺损坏死中再血管化与钙含量的关系
- Author:
Gang LIU
;
Wuxun PENG
;
Jin DENG
- Publication Type:Journal Article
- Keywords:
femoral head necrosis;
bone morphogenetic proteins;
basic fibroblast growth factor;
allogeneic bone
- From:
Orthopedic Journal of China
2006;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To observe the effect of composite(AACB/BMP/bFGF)of allogeneic antigen-extracted cancellous bone(AACB),bone morphogenetic protein(BMP)and basic fibroblast growth factor(bFGF)on the repair of defective and necrotic areas of the femoral head,and evaluate the revaseularization the femoral head defect and necrosis model using liquid nitrogen,and randomly divided into 3 groups with 12 femoral heads in each group,which were implanted with nothing(group A),AACB/BMP(group B)and AACB/BMP/bFGF(group C)respectively.Every femoral head was implanted with about AACB 0.33 g,BMP 12.5 mg and bFGF 2000 U.Two canines were sacrificed respectively at 3th,6th,and 12th weeks after operation in every group.Histologically,immunohitochemical vessel staining,vessel count and vessel surface area image analysis,calcium content and the correlation between revascularization effect and calcium content were investigated in the femoral heads after the operation.[Result]Histology,the detects were still infilled by fibrillar tissue in group,A;there were a great deal of new bone formed in group B;and the detects were repaired completely in group C 12 weeks after operation.Measurement of vessel count and vessel surface area showed a large quantity of new vessel proliferated around the infilling material in group C on the 3rd week.The vessel count and vessel surface area continuously increased on the 6th week and 12th week.They were more than those of group B and group A at every definite time point(P
