Microsphere Preparation of The Recombinant Adeno-Associated Virus as A Vector for Gene Therapy of Liver Cancer
- VernacularTitle:包载携有HSV-TK基因腺病毒PLG微球的制备
- Author:
Ziming LIU
;
Lnan YAN
;
Xinxiao LI
- Publication Type:Journal Article
- Keywords:
Gene therapy Biodegradable microsphere Adenovirus HSV-TK gene Liver cancer
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the suitability of the biodegradable microsphere encapsulation of adenovirus as a targeting vector for gene therapy of hepatocellular carcinoma. Methods Encapsulate the recombinant adenovirus in PLG poly (lactic/glycolic) copolymer by the solution evaporation method, the release test and the bioactivity of viruses incorporated in vitro were studied. Results More than 19.3% of adenovirus was encapsulated in PLG microspheres. The release test shows that the adenovirus was released for more than 200 h, 50% were shed within the first 100 h, and their activity was retained. Conclusion Recombinant adenovirus can be formulated in a polymer preparation of PLG with retention of bioactivity. It may be a valuable vector for the gene therapy of liver cancer.
