Effect of PTEN Gene in Regulation of Growth of Hepatocellular Carcinoma Cells by PPAR?
- VernacularTitle:PTEN基因在过氧化物酶体增殖物激活受体?调节肝癌细胞生长中的作用
- Author:
Hua XIAO
;
Feng ZHANG
;
Xuehao WANG
- Publication Type:Journal Article
- Keywords:
Peroxisome proliferators-activated receptor gamma Hepatocellular carcinoma PTEN gene
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of peroxisome proliferators-activated receptor (PPAR) ? on the growth of human hepatocellular carcinoma cells and explore the roles of phosphatase and tensin homologue deleted on chromosome ten (PTEN) and phospho-Akt in this process. Methods SMMC-7721 cells were treated with 15-d-PGJ2 or pioglitazone, which were two kinds of PPAR? ligands, at different concentrations. The viability of SMMC-7721 cells was evaluated by MTT assay. The cell cycle was analyzed by flow cytometry. PTEN mRNA level was determined by RT-PCR. The protein expressions of PTEN and pAkt were measured by Western blot analysis. Results It was demonstrated through MTT assay that both 15-d-PGJ2 and pioglitazone had an inhibitory effect on the growth of SMMC-7721 cells in a time- and dose- dependent manner. According to flow cytometry detection, more cells were arrested in G0/G1 phase. Increased expression of PTEN mRNA was detected in 15-d-PGJ2 or pioglitazone-treated cells through RT-PCR. Increased expression of PTEN protein and decreased expression of pAkt were confirmed by Western blot analysis. Conclusion The ligands of PPAR? could inhibit SMMC-7721 cells proliferation in a time- and dose- dependent manner. The upregulation of PTEN may be involved in the underlying mechanism.
