Effect of the PGE_2 Combined with TNF-? to the IP3-Ca~(2+) Pathway Mediated by Membrane TNF-?R of Fibroblasts
- VernacularTitle:前列腺素E_2与肿瘤坏死因子-?联合染毒对大鼠肺成纤维细胞增殖的影响
- Author:
Qiang GUO
;
Jiying JI
;
Suping LI
- Publication Type:Journal Article
- Keywords:
Respiratory system;
Fibroblasts;
Cytokines;
Second messenger in cell;
Tumor necrosis factor-?
- From:
Journal of Environment and Health
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of different prostaglandin E2 (PGE2) combined with tumor necrosis factor-?(TNF-?) to the IP3-Ca2+ pathway mediated by membrane TNF-?R of fibroblasts. Methods The lung fibroblasts of breed mouse were primarily cultured. 10 ?g/L TNF-? and 10 ?g/L TNF-? combined with PGE2 of different doses were added to culture medium of fibroblasts. At some observation time,the expression of TNF-?R on the cellular membrane of fibroblasts was detected by the method of immunohistochemistry,the level of IP3 in cells was detected by the method of radioimmunoassay and the contents of Ca2+ in fibroblasts by the method of flow cytometry. Results With doses of PGE2 increasing at 30 s time point and 60 s time point,the cpm value of IP3 of fibroblast decreased gradually. The cpm value of IP3 significantly increased when the dose of PGE2 was 1.0 ?g/L,but obviously lower when the dose of PGE2 was 2.0 ?g/L at 120 s time point. The expression of TNF-?R and the contents of Ca2+ decreased in a dose-dependent way with the dose of PGE2 increasing. Conclusion Certain dose of PGE2 could suppress the effect that TNF-? enhance the cell proliferation of fibroblast by the IP3-Ca2+ pathway mediated by membrane TNF-?R of fibroblasts.
