Role of Matrix Metalloproteinase-9 in Signal Transduction Pathway of Cigarette Smoke-induced Airway Mucous Hypersecretion
- VernacularTitle:基质金属蛋白酶-9参与吸烟所致气道黏液高分泌的信号转导通路
- Author:
Guoqing LI
;
Xiangdong ZHOU
- Publication Type:Journal Article
- Keywords:
Air pollutants;
Cigarette smoke extract;
Matrix metalloproteinase-9;
Epidermal growth factor receptor;
Mucin5AC
- From:
Journal of Environment and Health
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the involvement of matrix metalloproteinase-9(MMP-9) in the signal transduction pathway of cigarette smoke-induced airway mucous hypersecretion. Methods The well cultured BEAS-2B airway epithelial cells were incubated with different concentrations of cigarette smoke extract (CSE),also some cells were preincubated with MMP-9 inhibitorⅠbefore CSE or exogenous epidermal growth factor(EGF) was added. The mucin(MUC)5AC mRNA level was analyzed by RT-PCR,MMP-9 and the phosphorylated EGFR(p-EGFR) protein production was assayed by western blot,MUC5AC protein and EGF protein were detected by enzyme-linked immunosorbent assay respectively,and gelatin zymography was used to determine the activity of MMP-9. Results Incubation of BEAS-2B cells with CSE up-regulated MUC5AC gene expression and protein production in a dose dependent manner (P0.05). Conclusion CSE stimulates the activity of MMP-9 and MMP-9 protein,causing the shedding of the EGF and leading to EGFR activation. This activation of EGFR downstream signaling pathway increases MUC5AC gene expression and mucin production.
