Influence of RNA Interference Caspase-3 Gene on HEK 293 Cell Apoptosis Induced by Cadmium
- VernacularTitle:RNA干扰Caspase-3基因对镉诱导转化人胚肾293细胞凋亡的影响
- Author:
Yu WANG
;
Qinghui ZHANG
;
Wenjuan SUN
- Publication Type:Journal Article
- Keywords:
Cadmium;
RNA interference;
Caspase-3 gene;
Apoptosis
- From:
Journal of Environment and Health
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of RNA interference Caspase-3 gene on apoptosis of transformed human embryonic kidney 293 cells induced by cadmium in vitro. Methods Transformed human embryonic kidney 293 cell were treated with siRNA for 36 h. The treated cells were incubated with 40 ?mol/L CdCl2 for 12-24 h and the cells viability were measured with methyl thiazolyl tetrazolium(MTT) assay. In addition,the expression of caspase-3 gene in 293 cells was detected by the methods of reverse transcription polymerase chain reaction (RT-PCR) and Western-blot analysis,and the occurrence of apoptosis was determined by flowcytometry with Annexin-V and propidium iodide (PI) double labeling method. Results The results of RT-PCR and Western-blot analysis revealed that in incubated cells with 40 ?mol/L cadmium 12 h,the expression of Caspase-3 mRNA and 20 kDa protein significantly increased when compared with controls(n=4,P
