Effect of Aluminium on Concentration of Intracellular Ca~(2+) in Hippocampus
- VernacularTitle:铝对新生大鼠海马细胞内游离钙浓度的影响
- Author:
Rui REN
;
Baixiang LI
;
Yang ZHANG
- Publication Type:Journal Article
- Keywords:
Aluminium;
Calcium;
Hippocampus
- From:
Journal of Environment and Health
2007;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of aluminium on the concentration of intracellular Ca2+ in the hippocampus neurons of postnatal rat. Methods The hippocampus neurons of postnatal rats were primarily cultured and treated with Al3+ at doses of 0,10,100 and 1 000 ?mol/L. Fura-2-AM calcium ions fluorescence indicator was used to determine the concentration of the intracellular Ca2+ when they were cultured for 15 min,30 min and 45 min. Results The concentration of intracellular Ca2+ in the hippocampus neurons increased with the aluminum concentration increased and as the concentration of the aluminium was 1 000 ?mol/L,at the three cultured time points,the concentration of intracellular Ca2+ were (276.2?53.4) nmol/L,(306.5?73.7) nmol/L and (408.8?79.7) nmol/L respectively,significantly higher than the control group(P
