The immunological character of corneal epithelial cells
- VernacularTitle:角膜上皮细胞的免疫学特性
- Author:
Fan WANG
- Publication Type:Journal Article
- Keywords:
Antigen presenting cells(APC);
Corneal epithelial cells;
Peripheral blood mononuclear cells(PBMC);
Costimulatory pathway
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the capability of corneal cells to stimulate lymphocyte proliferation and the expression of costimulatory molecules on primary human corneal epithelial cells.Methods:Human corneal epithelial cells were cocultivated with peripheral blood mononuclear cells(PBMC). Expression of HLA class Ⅱ antigens, CD40, CD154, CD80 and CD86 was measured by immunohistochemical staining,and the expression of such molecules above on rejected corneal grafts was contrastingly assessed. Activation of lymphocytes was determined by measuring upregulation of CD69 by FACS analysis.Results:HLAⅡ expression was detectable on human primary corneal epithelial cells,and furthermore, upregualtion of costimulatory molecules CD40 and CD80 were also found after induction with ?-interferon. Both of HLA and CD40 were expressed on rejected corneal grafts. T lymphocytes were activated by epithelial cells in the coculture system.Conclusion:Human corneal epithelial cells are involved in rejection process of the corneal transplantation. The immunological reaction is triggered by epithelial cells,and endothelial cells suffer as the targets.
