The effect of Bifidobacterium BB12 on S.enteritidis-induced IL-8 secretion by Caco-2 cells
- VernacularTitle:双歧杆菌BB12对肠炎沙门氏菌诱导Caco-2细胞分泌IL-8的影响
- Author:
Ying HUANG
;
Yongchen ZHENG
;
Yan CAO
;
Chengye LU
- Publication Type:Journal Article
- Keywords:
Bifidobacterium BB12;
S.enteritidis;
Caco-2 cells;
IL-8;
NF-?B p65
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the in vitro effect of Bifidobacterium BB12 on IL-8 secretion by Caco-2 cells induced with S.enteritidis.Methods:The coculture systems of Caco-2 cells with S.enteritidis alone or combined with the strain of Bifidobacterium BB12 were established. The expression of IL-8 mRNA and NF-?B p65 in the cells were examined by reverse transcription PCR, real-time PCR and EMSA, and IL-8 in the supernatant was measured by ELISA.Results:In control group, there was little IL-8 mRNA expression in Caco-2 cells, NF-?B p65 was seldom in Caco-2 cells’ nuclei, and the concentration of IL-8 in the supernatant was as few as 126 ng/L. After stimulated by the S.enteritidis, the expression of IL-8 mRNA and activated NF-?B p65 was found in Caco-2 cells (P
