The correlation between point mutation of MBL ExonI and its plasma concentration in North Huis
- VernacularTitle:北方回族人MBL54位密码子基因多态性及血浆含量相关性
- Author:
Ping LI
;
Tianjun JIA
;
Wanyuan SHI
;
Rui HAN
;
Qiang LUO
;
Shumin ZHANG
- Publication Type:Journal Article
- Keywords:
MBL;
PCR-RFLP;
Huis;
Point mutation;
Concentration;
Relationship
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The major aim of this study is to analyze the point mutation at the codon 54th of MBL in healthy North Huis,and to measure the plasma levels of MBL, and to analyze the association between the mutation frequency and plasma MBL concentrations.Methods:PCR-RFLP was used to detect MBL point mutation.MBL plasma concentrations were measured using MBL Oliger ELISA kit.Results:Frequency of point mutation at the codon 54th of MBL in healthy Huis was 0.15. The plasma MBL concentration was (3.40?2.55)mg/L. There was negative correlation between MBL concentrations and gene mutation frequency in huis(r=-0.67).Conclusion:The relationship between frequency of mutation at codon 54 of MBL gene and the plasma MBL concentrations in healthy Huis is negative correlation.
