Effects of HMGA1 silence by RNA interference on the apoptosis and cell cycle of hepatocarcinoma cells
- VernacularTitle:RNAi沉默HMGA1基因对肝癌细胞凋亡和细胞周期的影响
- Author:
Mingguang ZHU
;
Xin ZHANG
;
Hongyan YUAN
;
Wei YANG
;
Yaping CHANG
- Publication Type:Journal Article
- Keywords:
RNA interference;
HMGA1;
Apoptosis;
Cell cycle
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop HMGA1-silencing SMMC-7721 cell and investigate its impacts on apoptosis and cell cycle in tumor cells.Methods:Constructing HMGA1-silencing SMMC-7721 cell through G418 selection of RNAi plasmid transfected cells;surveying the apoptosis,prliferation and cell cycle of the tumor cells by FACS and MTT.Results:Stable HMGA1-silencing cells were obtained successfully.The apoptosis percentage in RNAi group(29.46?3.04%) was significantly higher than that in the negative control (1.96?0.76%) or control (2.04?0.70%)within each P
