Effect of polypeptides from ehlamys farreri on human umbilical cord stem blood cells differentiation in vitro
- VernacularTitle:扇贝多肽对人脐血干细胞体外分化的影响
- Author:
Jianning LI
;
Xin SUN
;
Hao LIN
- Publication Type:Journal Article
- Keywords:
ploypetides from chlamys farreri;
human umbilical cord blood cells;
PTKs
- From:
Orthopedic Journal of China
2006;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To explore effect of polypeptides from chlamys farreri(PCF) on human umbilical cord blood cells(HUCBCs) differentiation in vitro,the effects of the extract on protein tyrosine kinase(PTKs) was studied in vitro.[Method]Getting human umbilical cord blood cells and cultured with the PCF at dilution concentrations of 1:1600,1:3200,1:6400,1:12800 nerve growth factor(NGF) was used as positive control and DMEM as negative one.After 48,72,96 h,the HUCBCs was cultured and the activity of protein tyrosine kinase(PTK) was examined by ATP in corporation test using PtK assay system.[Result]HUCBCs in the negative control group had no PTK activity,but that of positive control group was detected significantly.The PTK activity in extract of polypeptides from chlamys farreri groups increased at 48h(P
