Experimental study of recombinant human erythropoietin therapy for contusion injury of spinal cord
- VernacularTitle:重组人红细胞生成素治疗脊髓冲击性损伤的实验研究
- Author:
Bo WANG
;
Wei JIANG
;
Haiying LIU
- Publication Type:Journal Article
- Keywords:
recombinant human erythropoietin(rh-EPO);
spinal cord injury;
apoptosis;
ultrastructure
- From:
Orthopedic Journal of China
2006;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To study the therapeutic effect of recombinant erythropoietin for contusion injury of spinal cord.[Method]Contusion injury of spinal cord was caused by weight dropping in 24 New Zealand rabbits.Twelve hours after injury,the rabbits in control group were given natural saline intravenously and rabbits in low,mediate and large-dose group were given rh-EPO 100 IU/kg,500 IU/kg and 1000 IU/kg respectively.Neurological status of lower limbs were scored at 24 hours,48 hours and one week after spinal cord injury.All rabbits were killed one week after injury and spinal cords were stained by HE and caspase-3 method.Electronic microscopy was used to evaluate ultrastructural injury.[Result]The neurological scores of EPO treated groups were significantly higher than that of control group.HE and Caspase-3 immunohistochemistry showed that histological and ultrastructural damage of EPO treated groups were less severe than that of control group.The caspase-3 positive neurons were significantly fewer than that of saline treated group.There was no significant difference of therapeutic effect between mediate and large-dose EPO treated groups.[Conclusion]Rh-EPO administered 12 hours after contusion injury of spinal cord could lessen histological and ultrastructural damage,prevent apoptosis of neurons and promote neurological recovery of spinal cord.Mediate dose of rh-EPO is the appropriate treatment choice for spinal cord injury.
