The effect of TFPI-2 gene on apoptosis of pancreatic cancer cell
- VernacularTitle:TFPI-2基因在调控胰腺癌细胞凋亡中的作用
- Author:
Zhigang TANG
;
Zhenyang SUN
;
Hejie HU
;
Geliang XU
;
Jiong CHEN
;
Jiansheng LI
- Publication Type:Journal Article
- Keywords:
pancreatic neoplasms;
apoptosis;
tissue factor pathway inhibitor 2
- From:
China Oncology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose: TFPI-2 is a new serine proteinase inhibitor,it is related to many tumors.In this study,the effect of TFPI-2 gene on cell proliferation and apoptosis of human pancreatic cancer cell line Panc-1 were investigated.Methods:The growth curve was drawn for the 3 groups,Panc-1-TFPI-2、Panc-1-V and Panc-1.DNA strand breaks were used to detect the apoptosis of the 3 groups,and the change of cell cycle and apoptosis index was evaluated by flow cytometry.Results:Compared with nontransfected cells,the growth of Panc-1 cell transfected with TFPI-2 was inhibited significantly.The transfected cells showed a significant increase in G1/G0 phase.The apoptosis of Panc-1-TFPI-2 cell could be identified by DNA strand breaks and flow cytometry in comparison with the control group.The apoptosis index of the Panc-1-TFPI-2 cell(6.9?0.5)% was higher than the group Panc-1-V and group Panc-1[(3.0?0.4)% and(3.5?0.4)%]P
