A rat model of lumbar intervertebral disc degeneration by an anulus needle puncture
- VernacularTitle:大鼠腰椎间盘针刺退变模型的建立
- Author:
Liyang CUI
;
Shangli LIU
;
Yue DING
- Publication Type:Journal Article
- Keywords:
intervertebral disc;
degeneration;
animal model;
anular stab;
rat
- From:
Orthopedic Journal of China
2006;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To establish a reproducible rat model of disc degeneration by puncturing the annulus with needles of defined gauges.[Method]Twenty skeletally mature male Sprague-Dawley rats were used to determine the depth of puncture by measuring anular thickness of lumbar disc.Twelve rats were carried out transperitoneal ventral surgery and anular stab was performed using a 21-gauge needle to make a full-thickness or partial-thickness stab incision in the L3、4,L4、5 and L5、6 anteriorlateral disc.The L6S1 level was used as a control.Magnetic resonance images scans of the stabbed discs and control discs were performed at 4,8-week time point and after MRI histological analysis were performed.[Result]The depth of puncture was respectively partial-thickness 1.5 mm and full-thickness 2.3 mm.The stabbed discs exhibited a decrease signal intensity from T2-weighted images starting at 4-week time point in full-thickness stab incision and 8-week in partial-thickness.Histological analysis revealed disc degeneration in both stab incision groups at 4-week time point and full-thickness stab incision displayed more severe degenerative changes compared with that of partial-thickness.[Conclusion]Full-thickness or partial-thickness anulus stab incision with needles of defined gauges is reliable,effective and reproducible to establish a rat model of intervertebral disc degeneration.
