Study on the growth status and osteogenic potential of gene transferred mesenchymal stem cells with Ad-BMP-2 planted on tissue engineering scaffold
- VernacularTitle:转基因骨髓间质干细胞在组织工程承载体中生长及成骨转化的研究
- Author:
Guangrong JI
;
Meng YAO
;
Limin CHEN
- Publication Type:Journal Article
- Keywords:
BMP;
mesanchymal stem cells;
gene transfection;
tissue engineering
- From:
Orthopedic Journal of China
2006;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To observe the growth status and osteogenic potential of gene transferred mesenchymal stem cells with Ad-BMP-2 planted on allogenetic acellular and decalcified bone matrix served as tissue engineering scaffold.[Method]Rabbit mesanchymal stem cells transferred with Ad-BMP-2 were seeded on allogenetic acellular and decalcified bone matrix,the growth status of gene transferred MSCs on the scaffold was observed by scanning electron microscopy and the ostengenic potential was observed by testing contents of ALP and BGP and secretion of Collagen I in the culture supermatant.[Result]The MSCs transfected with Ad-BMP-2 grew well on the tissue engineering scaffold,the osteogenic ability of the gene transfected MSCs showed great significant difference compared with that of the control group.[Conclusion]The MSCs transfected with Ad-BMP-2 can grow well on the acellular and decalcified bone matrix and the osteogenic potential can be great improved by gene transfection.
