Development of a new type nerve tissue engineering scaffolds and its combination with bone marrow stromal cells
- VernacularTitle:新型神经支架材料的构建及其与骨髓基质干细胞复合的实验研究
- Author:
Hao MENG
;
Zhuojing LUO
;
Linjie FENG
- Publication Type:Journal Article
- Keywords:
tissue engineering;
collagen;
gelatin;
bone marrow stromal cells
- From:
Orthopedic Journal of China
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To develop a new type nerve tissue engineering scaffolds to repair the defect of peripheral nerve and observe the growth of bone marrow stromal cells cultured in vitro on the scaffolds.[Method]The biomaterial was made of I-collagen and gelatin by freeze-drying method,the alignment regularities of microscopic channels and there course directions were observed under the scanning electronic microscope.The size of the micropores and the factor of porosity were also measured.The bone marrow stromal cells were seeded on the collagen scaffolds and cultured for 5 days,then the growth of bone marrow stromal cells cultured in the scaffolds was observed.[Result]All the scaffolds were circular cylinder,the microscopic channels were arranged in parallel manners,and the pore sizes of the channels were uniform.The bone marrow stromal cells were seeded in the scaffolds successfully.[Conclusion]The developed nerve tissue engineering scaffolds have good structure and biocompatibility,which can be used in the repair of the nerve injuries.
